| One restriction enzyme allowed the identification of both minimus species, but also closely related species. |
| The restriction enzyme bound on DNA at the first site binds its second site to a remote DNA sequence and then dissociates from the first one. |
| The cDNA is digested with a restriction enzyme that cleaves the cDNA into fragments of approximately 256 base pairs. |
| The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed. |
| Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing. |
| If the restriction enzyme activities were inhibited, the DNA fragment would not be cleaved and should be seen in its original size on the gel. |
