| One restriction enzyme allowed the identification of both minimus species, but also closely related species. |
| Genetic characterisation of ASF virus isolates is achieved by determining restriction enzyme patterns and nucleotide sequences of portions of the virus genome. |
| Furthermore, sequencing data and Southern data indicated that no rearrangements of the DNA had occurred, as all internal restriction enzyme sites remained intact producing hybridization fragments of the expected size. |
| The restriction enzyme bound on DNA at the first site binds its second site to a remote DNA sequence and then dissociates from the first one. |
| The PCR-amplified products were confirmed as BHV-1 by restriction enzyme, Dde1, which produced fragments of predictable sizes, namely 340 and 128 base pairs. |
| If the restriction enzyme activities were inhibited, the DNA fragment would not be cleaved and should be seen in its original size on the gel. |
