| Biogen 1 discloses that the way to do it is to choose the restriction enzymes likely to cleave the Dane particle DNA into the largest fragments. |
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| Double-stranded oligos were multimerized using T4 DNA ligase in the presence of XhoI and SalI restriction enzymes to generate tandem arrays. |
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| In Southern blots, DNA is cut with restriction enzymes then separated. |
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| All constructs were verified by restriction enzymes analyses and sequencing. |
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| Eighteen candidate clones from the genomic library were digested with a series of restriction enzymes, Southern blotted, and hybridized with the same probe. |
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| The binding mechanism of psoralens to a double-strand DNA has been well investigated by chemical and enzymatic methods using restriction enzymes and exonuclease. |
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| For example, the fundamental research on the ability of bacteria to restrict incoming bacterial viruses led to the discovery of restriction enzymes, and the ability therefore to cut DNA at specific sites. |
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| Using proteins called restriction enzymes, individual genes from human DNA or genetically engineered in the laboratory, are isolated and inserted into small circular pieces of DNA, known as plasmids. |
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| These restriction enzymes cut DNA in specific locations. |
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| Golden Gate is a method that utilizes Type IIS restriction enzymes to allow scientists to assemble up to nine DNA fragments in a single step. |
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| A person must interfere in the process to obtain the required sequences of actions through many restriction enzymes. |
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| The result of the PCR is checked by electrophoresis after hydrolysis by two sets of restriction enzymes, making it possible to check the presence of both the GFP insert and the mutation. |
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| These DNA's, subjected to several restriction enzymes, show characteristic profiles after electrophoresis, specific to the varieties of the organism from which they originate. |
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| It goes back to early work in the medical labs in the U. S., where restriction enzymes, or molecular scissors, were developed to isolate and characterize. |
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| The plasmid DNA was analysed by digestion with restriction enzymes. |
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| A new idea is that the action of restriction enzymes will take place only when the transition molecule ligate to both ends of the circular molecule. |
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| Improved assessment of denitrifying, N2-fixing, and total-community bacteria by terminal restriction fragment length polymorphism analysis using multiple restriction enzymes. |
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| Each genomic DNA was having one or few sites for restriction enzymes. |
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| It was then digested with restriction enzymes, separated using gel electrophoresis, and transferred to a nitrocellulose membrane by a Southern blotting mechanism. |
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| Restriction enzymes covering all cleavable 4-bp palindromes can be used. |
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