| The cDNA is digested with a restriction enzyme that cleaves the cDNA into fragments of approximately 256 base pairs. |
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| Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing. |
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| If the restriction enzyme activities were inhibited, the DNA fragment would not be cleaved and should be seen in its original size on the gel. |
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| The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed. |
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| The restriction enzyme bound on DNA at the first site binds its second site to a remote DNA sequence and then dissociates from the first one. |
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| Analysis of all three nucleotide changes is performed using PCR amplification followed by restriction enzyme digest. |
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| The restriction enzyme and its corresponding methylase constitute the restriction-modification system of a bacterial species. |
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| One restriction enzyme allowed the identification of both minimus species, but also closely related species. |
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| Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. |
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| Genetic characterisation of ASF virus isolates is achieved by determining restriction enzyme patterns and nucleotide sequences of portions of the virus genome. |
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| Furthermore, sequencing data and Southern data indicated that no rearrangements of the DNA had occurred, as all internal restriction enzyme sites remained intact producing hybridization fragments of the expected size. |
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| The PCR-amplified products were confirmed as BHV-1 by restriction enzyme, Dde1, which produced fragments of predictable sizes, namely 340 and 128 base pairs. |
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| One restriction enzyme enables the identification of both An. minimus species. Moreover, using the same restriction enzyme closely related species could be distinguished as well. |
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| Genotyping was performed by restriction enzyme digestion of amplicons according to protocols provided by the supplier of the enzymes. |
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| The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining. |
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| For RFLP analysis HhaI restriction enzyme was selected. |
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| The restriction enzyme sites are underlined. |
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| The incubation at 50°C was performed to redigest and eliminate any plasmid that might still contain a BsaI restriction site, while the 80°C incubation was aimed at inactivating both restriction enzyme and ligase. |
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| They also contained restriction enzyme recognition sites and a myctag engineered at their 5' ends to facilitate subsequent synthesis of a plant transformation construct. |
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