How to use plasmid in a sentence

Looking for sentences and phrases with the word plasmid? Here are some examples.

Sentence Examples The plasmid DNA was then circularized with ligase and used to transform E. coli. The distribution of 2 plasmid DNA topoisomers was measured by Southern blot analysis with a radiolabeled 2 DNA probe. Molecular access to the sda gene was via the sda HZ.P1 mutation by the method of plasmid rescue of genomic DNA flanking the transposon insertion. We constructed plasmid fusions by cloning the promoter regions of the wild-type parent and the inversion mutant onto the pRS550 plasmid. Liposomal formulations of genetic drugs such as antisense oligonucleotides and plasmid DNA are difficult to achieve. Strains were transformed with a control centromeric plasmid or a centromeric plasmid carrying the wild-type GUK1 gene. Clearly, quenchable free radicals are the primary source of plasmid nicking in the purified solutions. The bacterium contains a plasmid, a circular piece of DNA that holds the gene and promoter sequence. For each transformation, the plasmid inserts from 7 to 18 colonies were sequenced. The researchers injected the DNA plasmid, which encodes a gene that stimulates the immune system, directly into the tumor site in mice. Consequently, the conjugational activity can be low when most cells carry the plasmid but increase greatly in response to plasmid-free cells. Twenty-four hours after transfection, the cells were harvested and the plasmid DNA recovered. Like bacteria, archaeans have no internal membranes and their DNA exists as a single loop called a plasmid. The same can also be expressed intracellularly when introduced through plasmid or viral vectors. Thus we were able to clone the DNA sequence that flanked the transposon insertion using plasmid rescue. Each step in the curing procedures was monitored by electrophoresis of plasmid isolations. Positive phage clones were converted to plasmid vectors using the material provided in the cDNA synthesis kit. The inserts were ligated into the plasmid vector and primary transformants were generated as per the supplied protocol. Cells transformed with a plasmid vector only were used as negative controls. The gene transference involves the use of a vector carrier which can be a plasmid or a virus. Show More Sentences An increase was seen in all 7 rabbits transfected with alkaline phosphatase plasmid. The process takes place in one direction since only the donor cells contain the conjugative plasmid. Plasmid DNA was prepared using the Qiagen plasmid purification system. A DNA plasmid comprising an adenoviral gene fragment E4 open reading frame ORF6 operably linked to an inducible promoter. We assume that focal gene transfer from chromosomals is extremely rare and that once the plasmid transfer occurs, the transconjugant will deterministically sweep to fixation. However, this type of plasmid still has the problem of segregational instability. Immobilization could also decrease the probability for segregational plasmid loss and overgrowth of plasmid-free cells. There was no incorporation of translatable plasmid DNA sequences outside of the T-DNA region. Once inserted into a plasmid, the gene can be glued in place using another enzyme called DNA ligase. What this means is that bacteria have an ability to transfer resistance through something called the plasmid. The genes that this plasmid contains then become incorporated into the genome of the plant. In animals studied to date the plasmid DNA does not replicate and is not integrated by host cells into their genomes. A plasmid containing this 3.1 kb of wild-type genomic DNA was cotransfected with a trp1 plasmid into protoplasts isolated from an auxotrophic thn1 mutant strain. The accuracy of each mutagenized plasmid was checked by sequencing. Biological containment involves crippling the plasmid vector and its bacterial host so that they will not be able to survive outside the laboratory. From 20,000 transformants screened, we isolated seventeen suppressors that were verified by isolating and retransforming the plasmid containing the histone mutation. The incubation at 50°C was performed to redigest and eliminate any plasmid that might still contain a BsaI restriction site, while the 80°C incubation was aimed at inactivating both restriction enzyme and ligase. The product was cloned into vector pGEM-Teasy, and serial dilutions of linearized plasmid were used to optimize the assay. Conjugation occurs when two bacteria come into physical contact with each other and a plasmid, sometimes carrying a piece of the chromosomal DNA, is transferred from the donor cell to the recipient cell. Cells were also electroporated with only plasmid DNA as expression control. Show More Sentences The plasmid DNA was then electroporated into competent Saccharomyces cerevisiae cells. They are located in a chromosome or a plasmid, and may be associated with transmissible genetic elements such as integrons or transposons, thereby enabling horizontal transmission from resistant to susceptible strains. Further, it is more economical than established reagent formulations currently available on the market for the introduction of plasmid DNA into CHO cells. This is where the automation comes in. The technology for moving a gene from one plasmid to another is not particularly new, but until now the process has had to be tailored individually to each gene. Then we ligated our gene into a pET plasmid that also contained a gene for resistance to ampicillin. The plasmid DNA was analysed by digestion with restriction enzymes. The resistance may be related to chromosomal, transposon or plasmid genes. Mosaic structure of a multiple-drug-resistant, conjugative plasmid from Campylobacter jejuni. This plasmid was first transformed into glycine auxotroph of E. coli strain and subsequently transformed into lysine auxotroph of Mycobacterium bovis BCG strain to generate vaccine BCG. HIVA2auxo. Presumedly, the continous production of the toxin becomes such a great metabolic burden to the cells, that the evasion by plasmid loss outweighs the initial toxication. This proprietary technology is based on the design of vectors carrying the genes of the immunoglobulin light and heavy chains on the same plasmid. This is how the F plasmid, which forms the basis of a lot of classical E. coli genetics, is transferred from one cell to another. Large-scale production of plasmid DNA for medical use is however a new field in biopharmacy. Association of the encapsulation of Bacillus anthracis with a 60 megadalton plasmid. Upon successful construction, the plasmid was transformed into bacteria to produce more plasmids. Recombinant colonies of C. sporogenes NCIMB 10696 were screened for the presence of the plasmid by PCR and also by retransforming the plasmid back into E. coli followed by restriction digestion verification of the plasmid. In contrast, the expression host itself, a DE3 lysogen induced with IPTG but harboring no expression plasmid construct, exhibited fairly uniform staining and morphology. Because of the diverse structures of the protein components of relaxasomes and transferosomes, the function of each plasmid protein cannot be definitively ascertained. The sequenceverified wild-type Bcl-2, mutated Bcl-2, and pLNCX2 empty vector plasmid were transfected separately into the BD RetroPACK PT-67 cell line. Recently we have developed cutting edge abilities to access a plasmid population of a given microbial community, and applied them to rumen microbial communities. Show More Sentences An Agrobacterium tumefaciens bacterial host containing the plasmid construct can then be used to insert the desired sequence into the plant genome. A silica spin kit for plasmid DNA minipreparation and a silica spin disc kit for plasmid DNA minipreparation containing silica matrix or discs respectively were from Biometra. We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. The biggest plasmid is highly homologous to a previously sequenced plasmid isolated from a horse and carrying additional multi-drug resistance genes. Plasmids have an essential impact on productivity. Related factors are plasmid copy number, structural plasmid stability and segregational plasmid stability. Examples from Classical Literature Glycoprotein B of B virus delivered in a plasmid vector induced a humoral response in both mice and rhesus macaques. Java of R-type ACSSpSuT from patients in England and Wales and Scotland were characterized by phage typing, plasmid profile typing, and pulsed-field gel electrophoresis. Find more words! 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Use our Sentence Generator Nearby Words plasmic plasmatic plasmas plasmapheresis plasma membrane plasmalemma plasmids plasmin plasminogen plasmodesmata plasmodia plasmodiocarp 7-letter Words Starting With p pl pla plas plasm plasmi