| This gives the appearance of directed mutagenesis because only revertant cells survive mutagenesis. |
|
| Such mutants have been generated by chemical mutagenesis, gene disruption, and antisense gene silencing. |
|
| A lower intensity of mutagenesis can explain all observed revertants if each mutagenized cell has multiple copies of the lac operon. |
|
| It has a wide cell tropism and remains extra-chromosomal, therefore minimising the risk of insertional mutagenesis. |
|
| In this way, ellagic acid effectively increases detoxification of carcinogens and reduces mutagenesis and tumourigenesis. |
|
| Sequence specific triplex formation can be applied for gene targeting, gene silencing and mutagenesis. |
|
| Plants for mutagenesis were the progeny of one pair of plants from this inbred population. |
|
| Evidence that PP may play a dominant role in UV-induced transition mutagenesis has been obtained for bacteria and lambda phage. |
|
| Similar arguments and requirements apply to mutants created by transposon mutagenesis. |
|
| Clofentezine was not mutagenic or genotoxic when tested in the Ames gene mutation assay and four other mutagenesis systems. |
|
| In the case of a virus the risk of insertional mutagenesis in mammal cells or the risk of carcinogenicity has to be discussed. |
|
| They developed the genetic tools to follow the fate of each cell, and produced many mutants by mutagenesis. |
|
| He has helped develop genomic methodologies in the area of structural genomics, ENU mutagenesis and genetics. |
|
| Kinetic assays under various conditions as well as mutagenesis and fluorescence assays will be used during those investigations. |
|
| He took a Nematode embryo in which ced-9, the natural protector, had been inactivated by mutagenesis. |
|
| Plant cells and tissues can be intentionally mutated through large-scale mutagenesis or mutation breeding to select model types. |
|
| A mutagenesis strategy autocatalytically converts mutations to the homozygous condition in fly somatic and germline cells. |
|
| To retain the AaargB sequence in the arginine auxotroph, the AaargB function was disrupted by insertion mutagenesis. |
|
| While the purpose of early mutant isolations was mostly focused on the identification of auxotrophs, many of these early strains have been subjected to heavy mutagenesis. |
|
| Aside from its value to basic research, site-directed mutagenesis has many applications in medicine, agriculture, and industry. |
|
|
|
| One method of in vitro mutagenesis is oligonucleotide-directed mutagenesis. |
|
| Smith first conceived of site-directed mutagenesis in the early 1970s and devoted several years to working out the details of the technique. |
|
| Moreover, the risk of insertional mutagenesis by using a retrovirus as a vector for gene delivery is unknown. |
|
| Novel plants that are developed using physical or chemical mutagenesis, somaclonal variation, embryo rescue, protoplast fusion or other methods producing wide genetic crosses, may also be included. |
|
| No evidence of mutagenicity was observed in an in vitro bacterial mutagenesis assay, a mammalian cell mutagenesis assay, or in an in vitro alkaline elution assay. |
|
| Smith remedied this situation by developing site-directed mutagenesis, a technique that can be used to modify nucleotide sequences at specific, desired locations within a gene. |
|
| This involves the investigation of structure-function relationships in glycosyltransferases and accessory enzymes, by kinetic analysis and structure determination, and mutagenesis for enzyme improvement. |
|
| The McGill University AIDS Centre, which he founded and still directs, was among the first laboratories worldwide to characterize the phenomenon of HIV escape from immunological pressure through mutagenesis. |
|
| However, after considering Canada's Novel Food Regulations, and hearing more about the application of such technologies as accelerated mutagenesis to crops like wheat, the discussion became drawn out. |
|
| Efforts are underway to verify the location of the second site by site-directed mutagenesis and the substituted cysteine accessibility method. |
|
| Emphasis was directed at chromosome 15, the target chromosome in the mutagenesis scheme. |
|
| Long-term studies in animals to evaluate carcinogenic potential and reproduction studies to evaluate mutagenesis or impairment of fertility have not been conducted with chloroprocaine. |
|
| More importantly, transgenesis has less impact on the expression of genomes or on protein and metabolite levels than conventional breeding or plant mutagenesis. |
|
| Haploidy breeding and mutagenesis for drought tolerance in wheat. |
|
| The drug combination was shown to work by lethal mutagenesis that could obliterate HIV by causing the virus to mutate to a point where it was no longer infectious. |
|
| The original ESR1 auxotrophs were created by transposon mutagenesis and assigned inferred genotypes based on their ability to grow on certain metabolic intermediates. |
|
