| Like bacteria, archaeans have no internal membranes and their DNA exists as a single loop called a plasmid. |
| The same can also be expressed intracellularly when introduced through plasmid or viral vectors. |
| Consequently, the conjugational activity can be low when most cells carry the plasmid but increase greatly in response to plasmid-free cells. |
| Each step in the curing procedures was monitored by electrophoresis of plasmid isolations. |
| Thus we were able to clone the DNA sequence that flanked the transposon insertion using plasmid rescue. |
| For each transformation, the plasmid inserts from 7 to 18 colonies were sequenced. |
