| Lavages were then performed at 10 minutes, 24 hours, and at 4 and 7 days afterward and analyzed for levels of IgE and histamine. |
| Data were classified as early or late responses, as well as with respect to whether the lavages were of the bronchial or alveolar regions of the lung. |
| We observed a washout effect with decreased biomarker concentrations after repeated lavages following exposure to air. |
| The acellular supernate from the first lavage was saved separately from subsequent lavages for analysis of lactate dehydrogenase activity and protein content. |
