We used perchloric acid because we found it much more effective at dissolving fluoride residues than either hydrochloric or nitric acid. |
The concentrations of the phospholipids were determined by phosphate assay subsequent to total digestion by perchloric acid. |
To this 1 ml of perchloric acid was added and the contents were mixed and allowed to stand for 5 minutes at room temperature. |
They found that these nonaqueous-superacid solutions reacted with weak bases which did not react with either sulfuric or perchloric acid in water. |
Simultaneously, hydrogen ions are produced, which react with the perchlorate ions making perchloric acid. |
Serum samples were deproteinized with 10 per cent perchloric acid, vortexed for 60 sec for mixing and centrifugation was done at 4000 rpm for 10 min. |