| The investigators derived the protoplasts from GAS by treating the GAS with phage lysin in a hypertonic medium, to prevent lysis. |
| This process leads to cell lysis and the loss of intracellular contents necessary for cell survival. |
| Inhibition of cell wall synthesis by penicillin resulted in disruption of the cell wall and lysis of the cell. |
| The patient was treated with methotrexate and leukovorin calcium, and tumor lysis syndrome with acute renal failure occurred. |
| This leads to late diagnosis, with progressive bone lysis affecting important circumjacent structures. |
| Recombinant plasmids were purified from overnight cultures using the alkaline lysis technique. |
