Inhibition of cell wall synthesis by penicillin resulted in disruption of the cell wall and lysis of the cell. |
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The osmotic gradient across the membranes can drive an influx of water, which causes the lysosomal lysis. |
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Marine amoebae are not in danger of lysis or crenation because seawater and their cytoplasm are isotonic. |
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Apparently cinnamaldehyde blocks mitochondrial respiration and causes lysis of cultured liver cells. |
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This process leads to cell lysis and the loss of intracellular contents necessary for cell survival. |
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The investigators derived the protoplasts from GAS by treating the GAS with phage lysin in a hypertonic medium, to prevent lysis. |
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All of these agents convert plasminogen to plasmin, which in turn breaks down fibrin and promotes clot lysis. |
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Upon asexual reproduction, the loricae of the ciliate kill the colonized area by emitting chemicals that cause lysis of the coral tissue. |
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Recombinant plasmids were purified from overnight cultures using the alkaline lysis technique. |
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An example is a transfusion reaction in which IgG and IgM anti-red-cell antibodies activate complement and cause cell lysis. |
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The patient was treated with methotrexate and leukovorin calcium, and tumor lysis syndrome with acute renal failure occurred. |
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This leads to late diagnosis, with progressive bone lysis affecting important circumjacent structures. |
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Presumably, the shock treatments that elicit bursting disrupt the balance of wall synthesis and lysis responsible for cell wall extension. |
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Plates can be examined for lysis at 24 and 48 h after inoculation with phage. |
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A partial or complete lysis of the long process of the incus was frequently associated, but in 9 cases it was the only cause of the failure. |
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Some nonclassical MHC molecules function as a ligand to a natural killer cell receptor and send a signal to prevent the cell lysis by the NK cell. |
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The lysis solution was poured on the slide to scrape off the cytological stained sample. |
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Instead, the droplet is first spun until the cell membranes rupture in a process called lysis, releasing the DNA inside. |
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Satisfactory recanalization was achieved by intraarterial lysis and the wound conditions improved. |
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The powder can then be thoroughly sonicated in lysis buffer to solubilize completely. |
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For example, one method requires soil to be added to a diatomaceous earth and lysis buffer, and then sonicated. |
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When working with fresh tissue, the cells must be sonicated immediately at the time the GITC lysis solution is added. |
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Plant cell suspension cultures and calluses can typically be sonicated in a lysis buffer within 2 minutes. |
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As agents that cause the lysis of fibrin clots, they are known collectively as fibrinolytic agents. |
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The fibrinolytic system that exists in the human body is also involved in the lysis, or dissolution, of clots as wounds heal. |
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Hyperuricemia frequently occurs in patients receiving thioguanine as a consequence of rapid cell lysis accompanying the antineoplastic effect. |
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The protease solution digests proteins to promote lysis, inactivates nucleases, and facilitates release of the RNA analyte. |
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Important: Do not allow the cells to stand in the lysis buffer for too long as they may also be lysed. |
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Severe lysis of Rh-positive red blood cells will occur at any subsequent transfusion. |
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If the antigen is on the surface of certain cells, activated complement can also facilitate the lysis of these cells. |
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This may cause the cells to form a gel and more lysis buffer may be added to reduce this effect. |
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This study furthers our knowledge of the malaria-parasite lysis in mosquitoes. |
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The morphological changes resulting from the PBP binding include filament formation or cell wall and septal thickening, and then cell lysis. |
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The active site of peptidoglycan lysis is disrupted in lactalbumin. |
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This lag could explain the degradation observed in samples from crude lysates produced by these lysis methods even in the presence of RNase inhibitors. |
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Then, they were thawed in 30 ml lysis buffer and immediately lysed. |
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However, apart from using an unstable enzyme, our first route had other problems, caused by cell lysis during the biotransformation, which hampered product recovery. |
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One class of dosage suppressors is composed of genes encoding cell wall proteins, indicating that alterations in cell wall structure can rescue the cell lysis in ram cells. |
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Tissues from gills, mantle, and digestive gland were individually homogenized in lysis buffer with a tissue tearor. |
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Some of these endolysins are secreted to the extracellular environment before completion of the viral lysis cycle independent of holin activity. |
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Since a high erythrocyte content will have a negative effect on the quality of the preparation, it is recommended that the erythrocytes be removed using a lysis buffer before derivation of the cyto preparation. |
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The washed P2 fraction was subjected to hypoosmotic shock and lysis before centrifugation again. |
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Metabolism and nutrition disorders: Tumour lysis syndrome. |
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The older medicine used to speak of two ways, lysis and crisis, one gradual, the other abrupt, in which one might recover from a bodily disease. |
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However, two loops of ileum were densely adhered to the anastomotic site and so the anastomosis was taken down with the lysis of adhesions. |
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Hydration, urine alkalinization, and prophylaxis with allopurinol to prevent hyperuricemia may minimize potential complications of tumor lysis syndrome. |
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Lot may have a problem that could cause an increased susceptibility for lysis of red cells from normal subjects. Yields uninterpretable results and causing the test results to not be generated. |
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To monitor the quality of DNA extraction and potential PCR inhibition, we added low concentrations of phocine herpesvirus to the lysis buffer. |
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A process for lysing liposomes, which comprises acting a lysis agent comprising a surfactant and avidin on a liposome membrane on which biotin has been previously affixed. |
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The lysis reagent causes lysis of the virus and nuclease inactivation. |
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Pegsiticase has received Orphan Drug designation from the FDA for refractory gout, tumor lysis syndrome and Lesch-Nyhan Syndrome. |
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The spheroplasts are then lysed with sonication in GITC lysis buffer. |
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What makes the results surprising is that in animal models, antibiotics cause bacterial lysis, leading to inflammation in the subarachnoid space. |
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Patients should be well hydrated and, when indicated, allopurinol should be administered for patients with high tumor load, to reduce the risk of tumor lysis syndrome. |
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If intravascular lysis occurred, complexes could continue to circulate. |
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Free mimivirus-like particles indicate egestion by amebae or the result of amebal lysis, a phenomenon observed in cocultures. |
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In cases of complete lysis of the long process of the incus, or in cases of a very short long process of the incus, a piston was put in the stapedotomy and attached to the malleus manubrium. |
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Therefore, in addition to lysis of an existing clot, BB-10153 may prevent reocclusion and reduce the need for administration of a separate antithrombotic agent. |
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Resolution of nephrocalcinosis associated with tumor lysis syndrome. |
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However, clinically defined respiratory events may be directly due Lo the leukemia-related involvement consist of leukostasis, leukemic infiltrates and lysis pneumopathy. |
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Babesiosis or tick fever, is a febrile disease of domestic and wild animals characterized by extensive erythrocytic lysis leading to anaemia, icterus and haemoglobinuria. |
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Lysis is caused by enzymes known as lytic enzymes that are secreted by the antibodies. |
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Lysis buffers based on chemotropic agents such as guanidine salts, however, interfere with subsequent hybridization and target capture. |
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