| The homogenate, obtained after sonication, was ultracentrifuged at 60 000 g for 30 min. |
| Combine in a sterile tube 5 ml of sample homogenate and 1 ml reconstituted EZTM Enzyme to give the desired final enzyme concentration. |
| The homogenate was incubated at room temperature for 5 min with agitation and then supplemented with 6 ml of chloroform. |
| Choose the concentration of homogenate that gives a substantial yellow color in about 5 minutes. |
| The homogenate was further macerated with quartz sand in a mortar with a pestle in a draft chamber. |
| Nasal turbinate homogenate from poults exposed to the 4 genotypes of hMPV was injected onto LLC-MK2 cells for virus isolation. |
