| Lipid hydration was coupled with six cycles of freezing, thawing, and sonication, except sonication was omitted in the last cycle. |
| The following day, cells were harvested by centrifugation, resuspended in buffer, and disrupted by sonication. |
| The pieces of tobacco should be visibly vibrating and the solution should be homogenous in colour at the end of the sonication. |
| The homogenate, obtained after sonication, was ultracentrifuged at 60 000 g for 30 min. |
| Chromatin was sheared by sonication, and DNA associated with either CLF1 or ORC1 was separately co-immunoprecipitated using an anti-HA antibody. |
| Cells were disrupted by sonication, and soluble recombinant proteins were purified on the TALON metal-affinity resin. |
