The rats were then treated with isoproterenol via the perfusate. |
We allowed flow to continue until less than 1 drop of perfusate drained from the circulation in 10 seconds. |
A subsample of the perfusate was refrozen for chemical analysis. |
After a further 15 min of perfusion with isosmolar perfusate, we added back leukocytes to the perfusate before instilling either acid or saline in the airway. |