In this paper we describe the tracking of single proteins in the cytoplasm and nucleoplasm of mammalian cells. |
Cells are partially fixed, lysed, and then extensively washed such that all cytoplasm and nucleoplasm are removed. |
It comprises the cell-protoplasm and a nucleus imbedded in it whose substance is known as the nucleoplasm. |
The gel-like matrix in which the nuclear components are suspended is the nucleoplasm. |
In young animals, the cytoplasm and nucleoplasm of most cells was smooth and uniform. |
Indeed, RpL5 has been shown to specifically bind 5S rRNA and transport it from the nucleoplasm to the nucleolus for assembly into the 60S ribosomal subunit. |