The photochemistry of ninhydrin in benzene and water was studied by laser flash photolysis and electron paramagnetic resonance. |
Total free amino acids in the leaf extracts and in the phloem exudate were analysed with the ninhydrin reagent. |
Standards were visualized by ninhydrin reagent and the chromatogram containing phosphorylated proteins was autoradiographed. |
In general the frequency of calibration runs depends on the stability of the ninhydrin reagent and the analytical system. |
The amino acids were separated in this HPLC-system by ion exchange and detected after post-column derivatization with ninhydrin at 570 nm. |
The proline content was determined by reacting with ninhydrin in an acid medium. |