| Urinary organisms such as proteus, providencia, and klebsiella with indoxyl sulphatase activity metabolise indican to produce indigo, particularly in alkaline urine. |
| The presence of ascorbate as a cosolute had little effect on visible emission, despite its demonstrated capacity to act as a strong reductant for the indoxyl radical. |
| Indol is absorbed and oxidized into indoxyl, which combines with potassium and sulphuric acid and is thus excreted. |
| Further oxidation and dimerization of indoxyl produces indigoid pigments. |
| Chemical Principle: This test pad contains an indoxyl ester and diazonium salt. |
| Organisms in the urine that possess indoxyl sulphatase activity metabolise urinary indoxyl sulphate to indigo leading to purple urine bag syndrome. |
