| When nothing is known about the mixture being separated, the column is prepared in ligroin or hexane, the least polar of the eluting solvents. |
| The hexane extract was evaporated, redissolved in chloroform and passed through a short silica gel column. |
| Solvents permitted in the UK for extraction include acetone, hexane, ethyl acetate, ethyl alcohol, and carbon dioxide. |
| The residue was redissolved in 0.5 ml of hexane and analysed by gas-liquid chromatography. |
| Distilled water, tap water and a solution of lead nitrate are extracted with a hexane solution containing dithizone. |
| In summary, the sample was denatured with aqueous ammonium sulfate and ethanol and was extracted with hexane. |
