| There is evidence that heating collagenous tissue causes tissue shrinkage through collagen denaturation. |
| When denaturation takes place, some of the physical and biological properties of the proteins are affected. |
| The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed. |
| This damage may occur in response to anything that will cause protein denaturation including thermal stress, osmotic stress, and chemical damage. |
| Therefore, a thermodynamic analysis of denaturation free energy change can be used. |
| Presumably, the increased chaperone need is induced by exaggerated gill protein denaturation in response to elevated body temperatures during emersion. |
