Serum samples were deproteinized with 10 per cent perchloric acid, vortexed for 60 sec for mixing and centrifugation was done at 4000 rpm for 10 min. |
The tube was vortexed for 2-3 min to dislodge conidia from the insect and the concentration of conidia was determined using a haemocytometer. |
The suspension was vortexed for 5 minutes, sonicated for 30 seconds and aliquoted into 100-litre samples. |
This residual volume was vortexed vigorously for 5 seconds at medium speed to resuspend the cells and cellular debris. |