| The ability of the seedlings to reduce triphenyl tetrazolium chloride was then determined, using the method of Steponskus and Lanphear. |
| The stains acridine orange, ethidium bromide, azur II, eosin B, trypan blue, Giemsa solution and nitroblue tetrazolium were purchased from Sigma. |
| The standard procedure for this assay was to remove the cell culture medium and add fresh media containing the MTS tetrazolium reagent. |
| Upon exhumation, we examined each replicate for germinants, rotted seeds, and whole firm seeds, which we tested for viability with tetrazolium. |
| After 24 and 48 h, aliquots were transferred to 96-well plates and incubated for 3 h with a tetrazolium salt. |
| The colonies grown on Compact Dry TC are red due to redox indicator tetrazolium salt. |
