This purification procedure provides a new, high efficiency method for the extraction of high purity rSLZ from culture supernate. |
The supernate was collected by magnetic separation for reproducibility test. |
The acellular supernate from the first lavage was saved separately from subsequent lavages for analysis of lactate dehydrogenase activity and protein content. |
After discharging in NaCl solution, metal contents in supernate and concentrated liquor were detected. |
Second Supernate cocktail from first infectivity trial, strains 149 and flag2 tested alone. |
The supernate of SMC incubated with DMSA-MNPs under the same experiment conditions was collected and mixed. |