| Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded. |
| Sonicate the capped vial for one minute or allow to sit for a minimum of one hour before analysis. |
| The sonicate was diluted 10-fold with IP Buffer to a final volume of 1 ml per immunoprecipitation reaction. |
| Add extraction solvent and mix or sonicate to dissolve. |
| Sonicate for a minimum of 10 minutes in an enzymatic detergent solution prepared per manufacturer directions. |
| The most effective means to resuspend the beads is to first vortex the bead suspension for approximately 30 seconds and then sonicate for approximately 30 seconds in a small bath sonicator. |
