| The enzymes involved in the breakdown of pectin include pectate lyase, polygalacturonase and pectinesterase. |
| It is a member of the PL1 family and is thought to have pectate lyase activity. |
| Several protein improvement strategies were used to improve pectate lyase PEL168 from Bacillus subtilis. |
| Using pectate lyase, Dr. Lau has found that certain amino acid substitutions can substantially affect either the thermostability or the catalytic activity of the enzyme. |
| Pectate lyase, a key enzyme in this process, has been genetically engineered for increased thermostability. |
| A cell comprising a DNA sequence coding for at least the following elements fused sequentially into one open reading frame, a pectate lyase, a target site for proteolytic cleavage, and a polypeptide of exogenous origin. |
