Current research focuses on optimizing experimental conditions for improved separation of monosome and polysome fractions in infected and control plant cells. |
The 80S monosome population was also decreased in IMPQ strain, but surprisingly, overall polysome level was rather similar between the two strains. |
Fractions corresponding to the monosome peak were collected and pooled. |
Three animals were used with separate preparations of monosome and polysomes per time point and individual microarray analysis. |