| It was not only possible to generate mother sporocysts from miracidia in vitro, but also to obtain adult worms from cercariae through in vitro cultivation. |
| Not only do P. sinitsini become adults in E. symmetrica, but eggs are produced and hatch inside the daughter sporocysts, releasing free-swimming miracidia. |
| Primary sporocysts were obtained after overnight axenic culture of miracidia as described. |
| Four hundred and fifty six molluscs were individually exposed to one miracidia. |
| Total RNA was isolated separately from miracidia, sporocysts, cercariae, schistosomulum, and male and female adult worms. |
| Neither SjIR-1 nor SjIR-2 was expressed in measurable levels in sporocysts or miracidia. |
