| This presents challenges, including the need to engineer the polygenic macronuclear genome, which has nearly 50 copies of each gene. |
| Negative controls were always performed with water or macronuclear DNA as initial template. |
| We have previously reported the existence of loci that assort together and hypothesized that these loci reside on the same macronuclear piece. |
| The micronuclei of wild-type pairs provided a reliable diploid standard to quantify the macronuclear DNA content of exconjugants by cytophotometry. |
| Note that macronuclear karyokinesis and cytokinesis has initiated in rad 51 nulls despite the failure to complete micronuclear mitosis. |
| A macronuclear pointer could derive entirely from the headpointer, entirely from the tailpointer, or chimerically from both, depending on the splice location. |
