This biosensor uses a genetically engineered strain of Escherichia coli specifically designed to produce a luminescent protein, luciferase, when it encounters dissolved arsenic. |
Nearly all of this light is produced by the oxidization of the molecule luciferin, a reaction catalyzed by the enzyme luciferase. |
The antibiotic-resistant colonies were isolated, and a second selection was performed to identify and isolate the transfectants with the highest luciferase expression. |
And that process, in turn, can be made visible using a second enzyme, called luciferase. |
A recombinant gene comprising an SOS gene espressed when a DNA is damaged and a gene expressing luciferase activity positioned downstream of the SOS gene. |
The light producing enzyme luciferase from fireflies is easier to detect than utrophin. |