| Mature spores have a two-layered wall, with both perispore and exospore, and their protoplast is fulfilled with lipid drops. |
| The rigid spore walls of these organisms are composed of two major layers, which are the exospore and the endospore. |
| Sometimes the exospore is ruptured and detached loosely from the germinating spore. |
| In the spore stage, the microsporidian cell is typically protected by a 30-40 nm thick proteinacious exospore and a 20-35 nm thick endospore layer. |
| Mature spores have a dense exospore coat, a pale endospore, and single rows of 8-11 polar tubule coils. |
| Immuno-electron microscopy revealed that this protein was expressed largely in endospore and plasma membrane during endospore development, but sparsely distributed in the exospore of mature spores. |
