| All assays were made in triplicate and possible interference with cross-reactive substances was checked by analysing a range of dilutions. |
| Serial dilutions of homogenized lungs were plated on Petri dishes containing trypticase soy agar. |
| Secondary antibodies, dilutions, and mounting conditions were as described above. |
| Samples were plated at varying dilutions on confluent Vero cell monolayers and observed for virus cytopathic effect. |
| Yes, but you need to have molecules present to cluster, which in typical homeopathic dilutions, is not the case. |
| Serial dilutions of the test solutions with culture medium were prepared before in vitro assay. |
