In this study we show how to determine with fluorescence anisotropy a quantitative value of the intrinsic resilience of the protein matrix. |
We observe a reduction in fluorescence anisotropy only in the presence of FRET from linked mVenus and mCerulean. |
The wavelength dependence reflects the degree and orientation of the optical anisotropy changes beyond 600 nm. |
Typically, the anisotropy decays of nanosecond decay time fluorophores are used to study the order and dynamics of the acyl side chain regions. |
It is still puzzling that the anisotropy and GP reported for laurdan do not show similar temperature-dependence profiles. |
The literature is full of other, more complicated ways to measure optical anisotropy, usually using a combination of polarizers and retarders. |