| In sequencing by ligation, a DNA ligase enzyme identifies the target sequence. this latter mettod is used in the solid sequencer. |
| The E.coli bacterium has an enzyme, DNA ligase, which can be used to repair these nicks in the sugar-phosphate backbone of DNA chains to form an intact double-strand. |
| The technique utilizes a thermostable DNA ligase to ligate together perfectly adjacent oligos. |
| Double-stranded oligos were multimerized using T4 DNA ligase in the presence of XhoI and SalI restriction enzymes to generate tandem arrays. |
| Restriction and modification enzymes as well as T4 DNA ligase were obtained from the same manufacturer. |
| This study shows that in a competition between the two enzymes, DNA ligase trumps the flap endonuclease in capturing bubbles and creating expanded tracts. |
