| To circumvent a possible problem of low data number, we pooled the observed and expected values for the three transposon families. |
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| Thus we were able to clone the DNA sequence that flanked the transposon insertion using plasmid rescue. |
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| Similar arguments and requirements apply to mutants created by transposon mutagenesis. |
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| We performed PCR on wild-type mouse genomic DNA to amplify sequences flanking transposon insertion sites for use as probes. |
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| Molecular access to the sda gene was via the sda HZ.P1 mutation by the method of plasmid rescue of genomic DNA flanking the transposon insertion. |
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| In the case of T-DNA and some transposon insertions there is no realistic possibility of reversion to wild type. |
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| In 2013, Gage and his colleagues compared transposon activity in ape and human stem cells. |
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| In fact, a number of brain disorders have been linked to elevated transposon events. |
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| The gus gene is derived from Escherichia coli and the nptII gene was originally obtained from the Tn5 transposon. |
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| This evidence is particularly strong in the parasitic blood fluke Schistosoma mansoni, in which Merlin is also the first described DNA transposon family. |
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| The nptII gene is derived from the prokaryotic transposon Tn5 and confers resistance to aminoglycoside antibiotics. |
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| Position-effect variegation results from the juxtaposition of euchromatin and heterochromatin by chromosome rearrangement or transposon insertion. |
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| A couple of the genes that silence transposon activity in humans do the same in chimps and bonobos, but we express them ten to twenty times more. |
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| Most, if not all, of these elements encode an enzyme called transposase, which acts to cleave the ends of the transposon, freeing it from its initial location in the genome. |
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| A DNA sequence designated as transposon Tn5401 containing an internal resolution site and having the nucleotide base sequence shown in Figure 1, or a mutant thereof capable of functioning as a transposable element. |
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| Repeated horizontal transfer of a DNA transposon in mammals and other tetrapods. |
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| The resistance may be related to chromosomal, transposon or plasmid genes. |
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| The nptII marker gene was originally obtained from Escherichia coli Tn5 transposon and is commonly used as a selectable marker for transformed cells. |
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| The original ESR1 auxotrophs were created by transposon mutagenesis and assigned inferred genotypes based on their ability to grow on certain metabolic intermediates. |
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| The demonstration that the short sleeper phenotype can be reverted in these lines by transposon jumping indicates that a single gene is likely to be involved. |
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