| The plates were then observed for the presence of the transconjugants, which were then biochemically identified. |
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| After overnight incubation the transconjugants were replica plated onto the same selective medium, omitting the other growth factors or substituting arabinose for glucose. |
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| Fitness estimates are complicated, in principle, by segregants and transconjugants that arise during competitions between plasmid-bearing and plasmid-free cells. |
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| The resulting plasmids were conjugated into the respective mutants of M. extorquens and the transconjugants tested for the ability to grow on methanol. |
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| Transconjugants were then selected on Luria-Bertani agar plates containing sodium azide and nalidixic acid. |
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