The amount of haemolysis was measured by comparing the absorption of the blood to that of the supernatant after sedimentation of the red cells. |
|
After 1 min of extraction, cells were sedimented and the supernatant was decanted. |
|
Cell debris was pelleted by centrifugation and supernatant concentrated by lyophilization to one tenth of the initial volume. |
|
The supernatant was used for soluble sugar determination and the last pellet was redissolved in distilled water for starch determination. |
|
The 10 ml samples were centrifuged and the absorbance of the supernatant was measured at 280 nm to determine the lignin content. |
|
At steady state, cells were centrifuged and the fluorescence of the supernatant measured. |
|
The supernatant was discarded and the pellets were dried in a desiccator over silica gel for 24 h and then extracted for the various assays. |
|
The supernatant was kept in stock whereas the residue was sequentially extracted three times. |
|
The supernatant was collected and the sediment suspended in a double volume of water, acidified and centrifuged. |
|
The tubes were centrifuged at 5000 rpm for 2 min, and the supernatant discarded. |
|
The resultant supernatant fraction was used for the determination of lipid peroxidation and glutathione. |
|
The supernatant was assayed for the determination of glycerol as just described for triglycerides. |
|
Ammonia N was determined on the supernatant fluid using the phenol-hypochlorite procedure. |
|
Fluorescence experiments after centrifugation confirmed that there were no vesicles in the supernatant. |
|
These were shaken for 1 hour, then the solutions were centrifuged and the clear supernatant was pipetted into vials. |
|
Ten minutes after adding malate, we again pipetted the supernatant from the same dish into other test tubes. |
|
Material was spun at 6000 rpm for 1 min to pellet nucleic acid, and supernatant was removed to a clean tube. |
|
The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected. |
|
After incubation, the samples were treated as above, and the supernatant fractions were lyophilized. |
|
The concentration of unbound protein in the supernatant was determined spectrophotometrically at 410 nm. |
|
|
Cell debris was removed by centrifugation, and the supernatant was fractionated by ammonium sulfate precipitation. |
|
The clear supernatant was loaded onto a Ni-chelating column, and bound protein was eluted with 500 mM imidazole. |
|
Cell debris was pelleted by centrifugation and the supernatant was tested for protein induction. |
|
The supernatant was desalted by centrifugal gel filtration and concentrated by ultrafiltration as previously described. |
|
Six milliliters of supernatant was transferred to a 30 mL separatory funnel and extracted with an equal volume of hexane. |
|
The erythrocytes were allowed to settle for 1 h, and the plasma, leukocytes and thrombocytes were separated by aspirating the supernatant. |
|
Finally, the excess lipid was eliminated by centrifuging the sample for 1 min, discarding the supernatant, rehydrating and vortexing the sample five times. |
|
The supernatant and the precipitate fractions were separated. |
|
Transfer a 20 ml aliquot of the supernatant to a suitable glass container and dilute with 20 ml water. |
|
Plasma, fresh-frozen' means the supernatant plasma separated from a whole blood donation or plasma collected by apheresis, frozen and stored. |
|
Antigen is present in the supernatant fluid at the end of virus growth but requires 50 to 100-fold concentration to be effective. |
|
Allow the sediment to settle and pipette 10,0 ml of the supernatant solution into a beaker. |
|
Sonicate and clarify, storing the supernatant at each stage, a total of three times. |
|
Depending on the approach, the sediment can be fully frozen and the supernatant derived quantitatively through simple decanting. |
|
Remove the fastening ring and the chamber. 4 Pipette off the supernatant carefully. |
|
The ammoniacal acetone supernatant containing extracted pigments was discarded, and the lipoprotein pellet was suspended in 2 mL of homogenization buffer. |
|
The supernatant was taken for spectrophotometric measurements. |
|
Effect of actinomycin on thymidylic kinase activities of 24-hour regenerating liver supernatant fluid. |
|
The infectious meal was prepared with fresh washed bovine red cells, viral supernatant and adenosine triphosphate at 5 mM as phagostimulant. |
|
The immobilization process was followed by the determination of the hydrolytic activity with p-nitro phenyl laurate of the supernatant. |
|
|
A 52±4 kD molecular weight protein, isolatable from a supernatant of the cell line M20-2, said protein being characterized by a pI of 4.1 ± 0.2 and an ability to inhibit or reduce an IL-1 mediated inflammatory response. |
|
Samples of the hydrolyzate were withdrawn every 1 h, centrifuged and the supernatant was analysed for reducing sugar. |
|
Centrifuge for 15 min at 3000 rpm and discard supernatant. |
|
Microfuge briefly to collect supernatant at bottom of tube. |
|
The cell lysate was centrifuged and the supernatant was used immediately in the kit. |
|
A lumbar puncture was done and the three tubes contained nonclotting blood and the supernatant fluid was xanchromatic. |
|
A model for the mechanism of this system hypothesizes that the peptide is secreted into the supernatant via the ABC-transporter. |
|
This aspect is clearly demonstrated by turbidity and bacterial counts taken from the supernatant and the sediments at the end of the test period. |
|
Upon storage, a white deposit and clear supernatant can be observed. |
|
Uronic acid content in each supernatant was assayed according to Blumenkrantz and Asboe-Hansen using galacturonic acid as standard. |
|
After successive rinsings with fresh water or seawater and removal of the supernatant with a pipette, only the larvae remain for microscopic examination. |
|
Two hundred and twenty five µlof putative virus-infected culture supernatant shall be added to each of two wells, mixed and 225 µl transferred to two further wells, i.e. a 1:5 dilution. |
|
After a further settling period of 10 minutes, 30 ml of the supernatant fluid is withdrawn by suction, leaving a volume of no more than 10 ml for examination in a petri dish or larval counting basin. |
|
The supernatant was subsequently used for the determination of extracellular enzyme activity. |
|
Sludge solubilisation indicated by increasing of SCOD in the supernatant. |
|
Furthermore, we present strong evidence that a pool of enzymatically active PfCK1 is secreted into the culture supernatant, demonstrating that PfCK1 is an ectokinase. |
|
The group used a centrifuge to separate the distillation remains of three kinds of shochu into solid bits and supernatant liquid, which they sterilized. |
|
The supernatant will be colorless and crystal clear in the case of traumatic tap and xanthochromic to hemolyzed if a pathologic bleed has taken place. |
|