How to use supernatant in a sentence

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Sentence Examples The amount of haemolysis was measured by comparing the absorption of the blood to that of the supernatant after sedimentation of the red cells. After 1 min of extraction, cells were sedimented and the supernatant was decanted. Cell debris was pelleted by centrifugation and supernatant concentrated by lyophilization to one tenth of the initial volume. The supernatant was used for soluble sugar determination and the last pellet was redissolved in distilled water for starch determination. The 10 ml samples were centrifuged and the absorbance of the supernatant was measured at 280 nm to determine the lignin content. At steady state, cells were centrifuged and the fluorescence of the supernatant measured. The supernatant was discarded and the pellets were dried in a desiccator over silica gel for 24 h and then extracted for the various assays. The supernatant was kept in stock whereas the residue was sequentially extracted three times. The supernatant was collected and the sediment suspended in a double volume of water, acidified and centrifuged. The tubes were centrifuged at 5000 rpm for 2 min, and the supernatant discarded. The resultant supernatant fraction was used for the determination of lipid peroxidation and glutathione. The supernatant was assayed for the determination of glycerol as just described for triglycerides. Ammonia N was determined on the supernatant fluid using the phenol-hypochlorite procedure. Fluorescence experiments after centrifugation confirmed that there were no vesicles in the supernatant. These were shaken for 1 hour, then the solutions were centrifuged and the clear supernatant was pipetted into vials. Ten minutes after adding malate, we again pipetted the supernatant from the same dish into other test tubes. Material was spun at 6000 rpm for 1 min to pellet nucleic acid, and supernatant was removed to a clean tube. The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected. After incubation, the samples were treated as above, and the supernatant fractions were lyophilized. The concentration of unbound protein in the supernatant was determined spectrophotometrically at 410 nm. Show More Sentences Cell debris was removed by centrifugation, and the supernatant was fractionated by ammonium sulfate precipitation. The clear supernatant was loaded onto a Ni-chelating column, and bound protein was eluted with 500 mM imidazole. Cell debris was pelleted by centrifugation and the supernatant was tested for protein induction. The supernatant was desalted by centrifugal gel filtration and concentrated by ultrafiltration as previously described. Six milliliters of supernatant was transferred to a 30 mL separatory funnel and extracted with an equal volume of hexane. The erythrocytes were allowed to settle for 1 h, and the plasma, leukocytes and thrombocytes were separated by aspirating the supernatant. Finally, the excess lipid was eliminated by centrifuging the sample for 1 min, discarding the supernatant, rehydrating and vortexing the sample five times. The supernatant and the precipitate fractions were separated. Transfer a 20 ml aliquot of the supernatant to a suitable glass container and dilute with 20 ml water. Plasma, fresh-frozen' means the supernatant plasma separated from a whole blood donation or plasma collected by apheresis, frozen and stored. Antigen is present in the supernatant fluid at the end of virus growth but requires 50 to 100-fold concentration to be effective. Allow the sediment to settle and pipette 10,0 ml of the supernatant solution into a beaker. Sonicate and clarify, storing the supernatant at each stage, a total of three times. Depending on the approach, the sediment can be fully frozen and the supernatant derived quantitatively through simple decanting. Remove the fastening ring and the chamber. 4 Pipette off the supernatant carefully. The ammoniacal acetone supernatant containing extracted pigments was discarded, and the lipoprotein pellet was suspended in 2 mL of homogenization buffer. The supernatant was taken for spectrophotometric measurements. Effect of actinomycin on thymidylic kinase activities of 24-hour regenerating liver supernatant fluid. The infectious meal was prepared with fresh washed bovine red cells, viral supernatant and adenosine triphosphate at 5 mM as phagostimulant. The immobilization process was followed by the determination of the hydrolytic activity with p-nitro phenyl laurate of the supernatant. Show More Sentences A 52±4 kD molecular weight protein, isolatable from a supernatant of the cell line M20-2, said protein being characterized by a pI of 4.1 ± 0.2 and an ability to inhibit or reduce an IL-1 mediated inflammatory response. Samples of the hydrolyzate were withdrawn every 1 h, centrifuged and the supernatant was analysed for reducing sugar. Centrifuge for 15 min at 3000 rpm and discard supernatant. Microfuge briefly to collect supernatant at bottom of tube. The cell lysate was centrifuged and the supernatant was used immediately in the kit. A lumbar puncture was done and the three tubes contained nonclotting blood and the supernatant fluid was xanchromatic. A model for the mechanism of this system hypothesizes that the peptide is secreted into the supernatant via the ABC-transporter. This aspect is clearly demonstrated by turbidity and bacterial counts taken from the supernatant and the sediments at the end of the test period. Upon storage, a white deposit and clear supernatant can be observed. Uronic acid content in each supernatant was assayed according to Blumenkrantz and Asboe-Hansen using galacturonic acid as standard. After successive rinsings with fresh water or seawater and removal of the supernatant with a pipette, only the larvae remain for microscopic examination. Two hundred and twenty five µlof putative virus-infected culture supernatant shall be added to each of two wells, mixed and 225 µl transferred to two further wells, i.e. a 1:5 dilution. After a further settling period of 10 minutes, 30 ml of the supernatant fluid is withdrawn by suction, leaving a volume of no more than 10 ml for examination in a petri dish or larval counting basin. The supernatant was subsequently used for the determination of extracellular enzyme activity. Sludge solubilisation indicated by increasing of SCOD in the supernatant. Furthermore, we present strong evidence that a pool of enzymatically active PfCK1 is secreted into the culture supernatant, demonstrating that PfCK1 is an ectokinase. The group used a centrifuge to separate the distillation remains of three kinds of shochu into solid bits and supernatant liquid, which they sterilized. The supernatant will be colorless and crystal clear in the case of traumatic tap and xanthochromic to hemolyzed if a pathologic bleed has taken place. Examples from Classical Literature On allowing them to stand, perfect subsidence took place, leaving the supernatant water colorless and brilliant. The supernatant ether, however, was slightly turbid in appearance, a fact which was at first ignored. Pour off the supernatant liquid, wash the precipitate with sugared water, express, mix it with 10 oz. A little of the supernatant ether is now removed to a small glass capsule or watch-glass, and allowed to evaporate spontaneously. You see here I can pour off the supernatant liquid clear, leaving our silver and gelatine as a clot at the bottom of the vessel. Plumbic acetate gives a purplish-red precipitate, mercuric cyanide a blue one, the supernatant liquid being also blue. The supernatant part is then to be poured off into a similar vessel. The mass is then boiled, when the supernatant oil is removed by skimming. Find more words! 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What is the opposite of supernatant? Words that rhyme with supernatant What is the plural of supernatant? Use our Sentence Generator Nearby Words supernally supernal supermundane supermoon supermom supermolecules supernatants supernate supernational supernatural supernaturalism supernaturalist 11-letter Words Starting With s su sup supe super supern superna supernat supernata supernatan