Such streaks do not yield confluent growth on medium without uracil, in contrast to prototrophic recombinants. |
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The cassettes were introduced into MR12 and yielded 15, 10, and 20 uridine prototrophic transformants, respectively. |
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A prototrophic control strain was also engineered. |
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Adenine prototrophic transformants were selected. |
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All the resulting strains were prototrophic. |
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