| Before analysis, cells were spun out of ethanol and resuspended in 1.5 ml PBS containing propidium iodide and RNase. |
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| We measured cell cycle distribution and apoptosis using propidium iodide staining and a flow cytometer as described previously by Mandil et al. |
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| Supravital exposure to propidium iodide identities apoptotic cells in the absence of nucleosomal DNA fragmentation. |
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| Three common flow cytometry dyes are fluorescin isothiocyanate, phycoerythrin, and propidium iodide. |
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| In addition, phalloidin staining of the actin cytoskeleton and propidium iodide uptake assays were performed to investigate cellular structural integrity. |
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