| Perhaps more important, excitation and photobleaching are limited to the focal region. |
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| The spot sizes of photobleaching can be changed using objectives of different magnifications and numerical apertures. |
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| Changes of state, photobleaching, and photocycling often involve subtle changes in tertiary structure. |
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| Another advantage of two-photon excitation is the decreased extent of photobleaching and photodamage above and below the focal plane. |
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| Their photobleaching behavior was studied using spectrally resolved emission spectroscopy. |
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| We found that fluorescence decayed with an averaged time constant of 142.8 s due to photobleaching. |
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| The porphyrin solutions were handled in the dark to avoid any photobleaching. |
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| The photoproduct again undergoes photobleaching during the second illumination. |
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| Unfortunately, it causes considerable photobleaching because the same fluorophores are under constant illumination. |
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| In addition, two-photon systems employ near-infrared light for excitation, which minimizes tissue damage, photobleaching, and intrinsic tissue fluorescence. |
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| For instance, the detection of the slow ejection kinetics may be impeded by the photobleaching effect and the possible diffusion of the probe through the permeable capsids. |
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| For photobleaching experiments, the iris of the microscope was reduced to the minimum diameter so that only a small region of the sample was excited. |
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