| The technique utilizes a thermostable DNA ligase to ligate together perfectly adjacent oligos. |
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| In the third step, an RNA oligo that carries two primer sites is ligated to the decapped mRNA by T4 RNA ligase. |
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| Double-stranded oligos were multimerized using T4 DNA ligase in the presence of XhoI and SalI restriction enzymes to generate tandem arrays. |
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| The plasmid DNA was then circularized with ligase and used to transform E. coli. |
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| A ligase catalyzing the formation of a carbon-oxygen bond between an amino acid and transfer RNA is called amino acid RNA ligase. |
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| Once inserted into a plasmid, the gene can be glued in place using another enzyme called DNA ligase. |
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| The E.coli bacterium has an enzyme, DNA ligase, which can be used to repair these nicks in the sugar-phosphate backbone of DNA chains to form an intact double-strand. |
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| Restriction and modification enzymes as well as T4 DNA ligase were obtained from the same manufacturer. |
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| This study shows that in a competition between the two enzymes, DNA ligase trumps the flap endonuclease in capturing bubbles and creating expanded tracts. |
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| In sequencing by ligation, a DNA ligase enzyme identifies the target sequence. this latter mettod is used in the solid sequencer. |
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| The glue is an enzyme called ligase, which stitches together loose sentences of DNA whenever it comes across them. |
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| The incubation at 50°C was performed to redigest and eliminate any plasmid that might still contain a BsaI restriction site, while the 80°C incubation was aimed at inactivating both restriction enzyme and ligase. |
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| Second, SCFTIR1 is an F-box ubiquitin protein ligase. |
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| This is accomplished by the application of an enzyme called DNA ligase, which seals the two segments, forming a continuous and stable double helix. |
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| If the Clearview Chlamydia MF result is negative and clinical symptoms persist additional follow-up testing is recommended e.g. using polymerase or ligase chain reaction. |
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| A polynucleotide ligase that effects the formation of the phosphate bond between adjacent sugar molecules is concerned with the repair function but may also have a role in synthesis. |
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| The research team headed by Jun Aruga has shown for the first time that a ligase named Rines regulates these levels. |
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| Erk activation is required for Nrf2 nuclear localization during pyrrolidine dithiocarbamate induction of glutamate cysteine ligase modulatory gene expression in HepG2 cells. |
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| Now, a research team headed by Jun Aruga at the RIKEN Brain Science Institute has shown for the first time that a ligase named Rines regulates these levels. |
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| Topoisomerases are enzymes with both nuclease and ligase activity. |
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| The first 2 platforms use sequencing-by-synthesis mediated by DNA polymerase, while the latter employs synthesis-by-ligation mediated by DNA ligase. |
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