| The tube was vortexed for 2-3 min to dislodge conidia from the insect and the concentration of conidia was determined using a haemocytometer. |
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| Triplicate wells of viable cells were counted using a haemocytometer and the experiment was repeated three times. |
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| Conidia in washing suspensions were counted using a haemocytometer. |
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| Counting was then done using the counting chamber in the haemocytometer. |
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| The cells were counted in a haemocytometer at 400X magnification. |
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| The sperm concentration was estimated using the Haemocytometer as described by Salisbury et al. |
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