| The coverslips were pretreated with sterile poly-L-lysine to improve cell adhesion to the glass. |
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| The samples were then prepared on concave microscope slides under coverslips and sealed with silicone grease. |
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| Cells were seeded onto glass coverslips at a density of 1 X 101 cells per dish, and allowed to attach. |
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| After electroporation, cells were plated on glass coverslips submerged in maintenance media. |
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| All measurements were performed on uncoated glass coverslips, avoiding errors in the viscoelastic data due to a soft substrate. |
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| The sections were then counterstained in eosin, dehydrated, cleared, and mounted with glass coverslips. |
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| The silanized coverslips were held in a glass beaker covered with aluminum foil for up to 5 days. |
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| The root sections were mounted under coverslips using an anti-fade mountant. |
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| Slides were counterstained with hematoxylin, dehydrated, and mounted with glass coverslips. |
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| After a final wash, coverslips were mounted in Vectashield mounting medium. |
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| The slides were finally mounted with coverslips and coded for analysis to avoid bias. |
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| Two days after transfection had been initiated, cells were passaged onto coverslips. |
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| For AFM experiments, the cells were plated on presterilized coverslips a day before data were taken. |
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| Cells were plated on sterile coverslips placed in 60 mm culture dishes, using the same suspension density as the one used in the MTS assay. |
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| No counterstain was used on the sections, but routine coverslips were applied. |
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| Glass slides and coverslips are not recommended because they do not produce reliable, consistent results. |
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| Flow cells were fabricated by assembling two glass coverslips separated by a thin layer of parafilm. |
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| Glass coverslips were washed with ethanol followed by distilled H2O and allowed to air dry for 30 min before sample adsorption. |
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| Exponentially growing cells were plated in a 6-well plate on glass coverslips and cultured for 24 h prior to drug treatment. |
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| In this work, we have immobilized eqFP611 on glass coverslips coated with polymer chains. |
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| Polyacrylamide gel samples were prepared on aminosilanized glass coverslips using published methods. |
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| We would like to mention that we found a different behavior in fibroblasts on laminin-coated glass coverslips. |
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| The coverslips were mounted onto glass microscope slides with the addition of 3 l of mounting medium. |
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| The mucus trails were protected with coverslips and observed under a light microscope. |
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| Glass coverslips were adhered to the petri dish by placing them over each water drop. |
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| Before use, the microcells and glass coverslips were cleaned with piranha then rinsed thoroughly in de-ionized water. |
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| Borosilicate glass slides and coverslips were used in all imaging experiments. |
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| For the experiments, cells were grown on glass coverslips for several days. |
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| The tumor cell line was directly cultured on the coverslips. |
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| The coverslips were mounted, cell side up, on labelled microscope slides. |
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| We attempted to mimic a more biological situation by studying cells embedded in confluent monolayers which are grown on collagen or microcellulose-coated glass coverslips. |
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| Coverslips were then coated with gold and attached to an SEM stub with tape or rubber cement before SEM scanning. |
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