As the force is increased, the structural parameters of the DNA double helix are revealed, including the free energy of the basepair interaction. |
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The enthalpy, entropy, and free energy changes in the opening reaction of each basepair are determined from the temperature dependence of the exchange rates. |
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This ensures that no basepair in the DNA double helix, regardless of its sequence context, acquires a very high thermodynamic propensity for opening. |
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