| This included a separate area for pre-PCR manipulations, dedicated air-displacement pipettes, barrier tips, and aliquoted stocks. |
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| Where appropriate a master mix of PCR reaction components was made and aliquoted into individual reactions. |
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| After 10 min of incubation at room temperature, 1.25 ml of solubilized extracts were aliquoted into 1.7-ml microcentrifuge tubes. |
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| The IP pellet was resuspended in final wash buffer and aliquoted for subsequent biochemical analyses. |
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| Following etherization, flies were aliquoted into groups of 20, placed into vials, and allowed 1 day for recovery from etherization. |
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| From each patient, 6-10 ml of venous blood was drawn and aliquoted in plain tubes without anticoagulant. |
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| The suspension was vortexed for 5 minutes, sonicated for 30 seconds and aliquoted into 100-litre samples. |
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| A unit of packed red blood cells aliquoted into two bags was ordered for transfusion. |
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| The solution was then aliquoted into individual 435-l volumes. |
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| Reagents were aliquoted and each aliquot was used only once. |
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| The DNase-treated RNA was precipitated, quantitated and aliquoted. |
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| The inoculum was aliquoted into sterile, 8-mL, screw-cap glass vials. |
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| Spiked samples were mixed gently but thoroughly, aliquoted into 20 ml glass scintillation vials and placed into the photosynthetron. |
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| We suggested the creation of a common specimen bank for normal reference studies with adequate numbers of aliquoted samples to enable widespread distribution. |
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| An aliquoted FOXL2 PCR product was used for bidirectional DNA sequencing. |
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