How to use PCR in a sentence

Looking for sentences and phrases with the word PCR? Here are some examples.

Sentence Examples In addition, PCR can detect small deletions and can specifically localize the regions contained in the deletion. Different numbers of PCR cycles were performed to determine the logarithmic phase of the reaction. We performed PCR on wild-type mouse genomic DNA to amplify sequences flanking transposon insertion sites for use as probes. Pools of all sizes tested gave positive signals indicating that all the pool sizes are amenable for the PCR assay. Where appropriate a master mix of PCR reaction components was made and aliquoted into individual reactions. Similar results were obtained from at least two independent PCR assays of two independent chromatin isolations. The DNA sample from the patient is labeled and amplified by PCR and then added to the filter paper. The positions of primers used for PCR amplification and sequencing and the diagnostic PstI site are indicated. The junctions of the rearrangements were then amplified by PCR and sequenced to reveal the breakpoints. Three randomly picked clones from each PCR reaction were eventually sequenced. In cases where PCR recombination was suspected to occur uniquely in a single clone, the mosaic clone was removed from consideration. Thirty microlitres of PCR product was loaded on 1.5 per cent agarose gel electrophoresed and stained with ethidium bromide. We performed two experiments in order to eliminate the possibility that the recombination we observed was an artifact of the PCR reaction. Arrows below the gene map indicate primers used in PCR analysis of the P-element insertions. After genetic isolation of P insertion chromosomes, the flanking sequences were obtained by inverse PCR and sequenced. The two loci marked with a number sign indicate those analyzed by quantitative PCR in the previous study. Immunoprecipitation, deproteinization, and PCR were performed as described. Species-specific primers were developed to amplify the PBP gene region, and three individuals were sequenced directly from these PCR products. Their portability from bread wheat to durum wheat has been verified by PCR amplification in durum wheat, using primers defined for bread wheat under the same PCR conditions. After construction of the chimeras by overlapping PCR, all of the chimeric genes were cloned into integrating vectors under control of the SEC9 or the SPO20 promoter. Show More Sentences The assay was based on the incorporation of digoxigenin labeled dUTP and a biotin labeled primer specific for Shiga toxin genes during PCR amplification. This seems particularly likely in the patient who had an old, inactive hip joint tuberculosis and a false-positive PCR test result from the knee joint sample in this study. In 17 of 19 cases PCR yielded nonspecific products or failed. The PCR products were analyzed by electrophoresis on agarose gels containing ethidium bromide and were photographed under ultraviolet transillumination. The fourth case failed to demonstrate clonality by flow cytometry and PCR owing to a lack of viable cells and lack of amplification, respectively. The EBV genome and clonality were detected by Southern blot and PCR assay. Probes were obtained by PCR amplification of the indicated loci. These instruments also have built-in fluorimeters that detect fluorescence emitted from nucleic probes annealed to target DNA at the conclusion of each PCR cycle. In conclusion, PCR can be used as an additional test for detecting M. tuberculosis in patients with tubercular pleuritis because conventional methods have low sensitivity. Absence of the wild-type gene was also verified by PCR at this point. For MHC sampling, degenerate PCR primers were designed from an alignment of guppy, topminnow, cichlid, and bass Mhc class II B sequences found in GenBank. Detection of Chlamydia trachomatis from urine specimens by PCR in women with cervicitis. We also performed PCR to amplify a portion of D loop using the same primers and thermoprofile used by Bozarth et al. It is classified into PCR, sequencing, northern blotting, southern blotting, molecular cloning, and other nucleic acid applications. Purity of DNA was checked and contamination by bacterial DNA was ruled out by performing a 16S ribosomal DNA PCR for eubacteria. Real-time PCR for detection and quantification of the protistan parasite Perkinsus marinus in environmental waters. The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining. For the analysis of the COL4A4 gene, all the exons including splicing sites were amplified by PCR and screened by direct sequencing analysis. Diagnostic immunohistochemistry and PCR are available in only a few reference laboratories. A repeat vitreous tap in April 2011 showed a positive PCR for VZV in addition to CMV and she received 2 weeks of intravenous acyclovir. Show More Sentences A multiplex PCR assay was used with primers used for the K99, Sta, Stx1, Stx2, intimin, and the F41 fimbrial subunit genes. On PCR testing of the ingluvies and surrounding tissues, results were positive for Aspergillus species. The extracted DNA was then subjected to 18S rDNA PCR for free-living amebas as described by Tsvetkova et al. Use of real-time PCR for determining copy number and zygosity in transgenic plants. Multiplex PCR for typing and subtyping influenza and respiratory syncytial viruses. Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis. All fungi were identified as Ascomycetes based on their ITS region of rRNA using PCR followed by DNA sequencing. Multiplex PCR targeting tpi, tcdA, and tcdB genes for toxigenic culture of Clostridium difficile. The successful PCR amplicons were sent for direct sequencing to the Chromous Biotech Pvt. As such, FISH and PCR complement each other and are both essential for detecting t translocation. Recently, 3rd generation PCR technology, named digital PCR, has developed for directly quantifying and clonally amplifying nucleic acids. Detection of Neisseria meningitides in clinical samples by a duplex real-time PCR targeting the porA and ctrA genes. Microarrays, PCR and RNAi, have been gaining prominence in this microtechnology era. To monitor the quality of DNA extraction and potential PCR inhibition, we added low concentrations of phocine herpesvirus to the lysis buffer. In addition to identifying uncultivable organisms, PCR and RT-PCR assays have the advantage of obtaining quick results, often within 24 hours. The 5' nuclease assay uses fluorogenic allele-specific detection probes that allow PCR amplification and allele detection in a single procedure. Comparison of PCR assay and cytotaxonomy for identification of Anopheles fluviatilis sibling species. Real-time PCR assay for detection and relative quantification of Liocarcinus depurator larvae from plankton samples. The web site walks the researcher through the design of the primers needed for conducting allele-specific PCR against the SNP target of interest. The GSTP1 polymorphism was determined by a tetra-primer amplification refractory mutation system PCR as described in an earlier study. Show More Sentences Simple and sensitive detection of mutations in the ras proto-oncogenes using PNA-mediated PCR clamping. Development of novel real-time PCR assays for detecting DNA virus infections in psittaciform birds. Identification of Pythium insidiosum by nested PCR in cutaneous lesions of Brazilian horse and rabbits. One strand is usually overproduced by asymmetric PCR to allow intramolecular hairpin formation and genotyping using the excess strand. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. Last month's Primer dealt with endpoint product detection methods for PCR, with a primary focus on the agarose gel electrophoresis method. The axenicity of the filtered samples was proven by PCR using the primers and cycling conditions described by Spoerner et al. The research will use Orion's proprietary DNA methylation technologies, including MethylScope microarrays and MethylScreen PCR assays. These parameters further support the usefulness of the new heptaplex PCR assay in routine clinical diagnosis and infection control. A significant proliferation of the infectious agent does not occur, this limits the ability of PCR to detect the presence of any bacteria. A real-time RT quantative PCR for the detection of bovine ephemeral fever virus. The high degree of variability posed problems for the design of PCR and sequencing primers. Repetitive element PCR fingerprinting using enterobacterial repetitive intergenic consensus primers is not necessarily directed at ERIC elements. To enhance the samarium signal, especially after the blood-spot PCR, polyethylene glycol and dextran sulfate additives were tested. The assay is based on a combination of multiplex PCR and biochip array hybridization. Thousands of samples can now be sorted by this PCR alone without needing Southern blot or capillary electrophoresis. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adaptors chosen at random from a total set of 9,216 molecular indices. The eluent typically flows to a PCR amplification zone after purification. Results of reverse transcription PCR were negative for WNV and members of genus Flavivirus in serum and CSF samples taken 4 days after disease onset. We describe 2 cases of serogroup C meningococcal disease diagnosed post mortem by PCR from vitreous humor and immunohistochemical staining of tissues collected at autopsy. Show More Sentences Where possible, archived DNA from family members was tested with PCR, restriction endonuclease or sequence analysis for the putative change identified in the proband. Direct PCR detection of phytoplasmas in experimentally infected insects. Actually, when the STR loci in question are on an autosome as most are, then a human DNA sample will have two loci copies, so two PCR products will be amplified. Like PCR, however, this pairing is driven by sequence homology, and if the correct matching target sequence is not present, the T7-tagged primer will not successfully anneal. Digital PCR is a novel application of the now 30-year-old PCR technology. Lymph node sampling by fine-needle aspiration showed caseating granulomatous inflammation, but AFB and fungal smears and Mycobacterium tuberculosis PCR results were negative. Techniques such as PCR, DNA microarray, DNA sequencing, mass spectrometry and flow cytometry have driven discoveries that have changed our understanding of the world. The clone libraries of the spirochete-specific PCR products showed that clones retrieved from all investigated bivalve species matched sequences of the spirochetal group. In particular, advanced real time PCR technology is increasingly being used in the rapid detection of food pathogens and spoilage micro-organisms. Bacterial mycobacterial and mycotic cultures of the CSF, PCR analysis for Herpes Simpelx Virus and neurotropic viruses in the blood and CSF were negative. Briefly, red blood cells are first lysed in a large volume, which dilutes the hemoglobin and anticoagulants to an extent that PCR and post-PCR treatments are not inhibited. The present study aims to design and test a nested PCR system that targets the COI gene in order to identify the RTB from the four other beetles in the bark beetle family. As a result, extension rates better reflect the kinetics seen in PCR with processive extension of a defined template for more reproducible activity measurements. An aliquoted FOXL2 PCR product was used for bidirectional DNA sequencing. Reverse transcription PCR was conducted using a primer pair targeting nonstructural protein 1 of Getah virus by using the RNA extracted from the blood samples. All strains were positive for fcpA, tcpI, acfB, toxT, ctxA, zot, and ToxR genes, as well as for the O139-specific genomic DNA in DNA probe or PCR assays. Third, the essential tools for directing PCR, primers, are derived from the genomes of infectious agents, and with time those genomes will be known, if they are not already. Oligonucleotide primers for PCR amplification of coelomate introns. The only remaining blockades to the use of PCR as a standard tool of diagnosis are in its cost and application, neither of which is insurmountable. Examples from Classical Literature In the late 1960s the PCR was disturbed by the extent to which abstract art had blossomed despite party disapproval. GoTaq 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR method. Two experienced microscopists at the Amani Laboratory in Muheza, Tanzania, read the slides blinded to the PCR or RDT results. From the differences between cranberry tipworm and blueberry gall midge sequences, diagnostic PCR primer sets were developed that distinguish the two populations. Reverse transcriptase PCR does n ot unambiguously discriminate between living and dead cells, as all traces of DNA must be removed for the technique to be reliable. Find more words! 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What is the past tense of PCR? What is the plural of PCR? Use our Sentence Generator Nearby Words PCP PCOS PCO PCM PCI PCC pcs PCs PCS PCSO pct PCV