| The number of cells with chromosomal aberrations among 100 well-spread metaphases was recorded. |
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| Cells were classified with regard to the presence of abnormal metaphases and aberrations of any of the stages of mitosis. |
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| During pachytene, the chromosomes are less condensed and much longer than in mitotic metaphases. |
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| At later periods, extremely aberrant metaphases predominated. |
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| After three subsequent changes of fixative, metaphases were prepared by dropping the cell suspension onto labelled clean, moist microscope slides. |
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