During metaphase in both animal and budding yeast cells, chromosomes exhibit directional instability, moving first one way and then the other. |
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Two pairs of loci were observed in each somatic cell at metaphase and interphase. |
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Signals from both subfamilies were mainly located in the centromeric regions of maize metaphase chromosomes. |
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The events in this stage of the cell cycle leading to cell division are prophase, metaphase, anaphase and telophase. |
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In metaphase, sister kinetochores attach to microtubules emanating from opposite spindle pole bodies during alignment of the chromosomes. |
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A critical step in the cell cycle is the proper attachment of chromosomes to the mitotic spindle during metaphase. |
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The metazoan centromere was first identified cytologically as the region of the primary constriction of a metaphase chromosome. |
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True non-disjunction as a mechanism causing aneuploidy was separately assessed when a bivalent was found in a set of metaphase II chromosomes. |
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The three other products of female meiosis, the polar bodies, arrest in a metaphase state and associate with radiating arrays of microtubules. |
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These tests score either chromosomal structural aberrations at metaphase or micronuclei at interphase. |
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Chromosome loss takes place by lagging of chromosomes at metaphase, for example because spindle fibres may fail to attach to a chromosome. |
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Chromosomal damage can be assessed by the cytogenetic analysis of chromosomes at metaphase. |
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Chromosome configurations at metaphase I of two triploids and two aneuploids were comprised of univalents, bivalents, and trivalents. |
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The most direct way to assess chromosomal aneuploidy is to evaluate chromosome numbers in metaphase cells. |
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The problem is greatest at mitotic metaphase, when chromosomes are shortest and neighboring signals are thus closest. |
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One hundred randomly selected metaphase cells were scored for the presence of chromatid gaps and breaks. |
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Chromosome aberrations were scored on 50 metaphase cells per clone. |
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In metaphase, chromatid fragments are attached to the homologous area of the sister chromatid while acentric chromosome fragments are apart from their chromosome of origin. |
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Figure 2 Mitotic and Meiotic metaphase plates and karyogram of regenerated Aloe vera plants. |
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Cells were plated on a cross shaped adhesive micro pattern and retraction fibers were ablated on one branch of the cross in early metaphase. |
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The chromosomes, each of which is a double structure consisting of duplicate chromatids, line up along the midline of the cell at metaphase. |
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In metaphase the condensed chromosomes align in a plane across the equator of the mitotic spindle. |
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Chromosomes are best seen at the point in cell division called the metaphase stage of mitosis. |
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They are then stopped when they reach a step in cell division called metaphase. |
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Out of 180 embryos processed, 109 had at least one analyzable metaphase. |
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The pilot study shows that in vitro grown and matured oocytes from preantral follicles that progress to metaphase II possess euploid numbers of chromosomes. |
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At metaphase, centrosomes initiate the bipolar microtubule spindle. |
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During late anaphase and telophase, the metaphase furrows rapidly regress. |
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To summarize, metaphase spreads were made from a 24-hour, unstimulated cell culture and were G-banded by the standard trypsin-Giemsa banding method. |
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At least 10 spermatocytes at the metaphase I stage were recorded per male. |
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We karyotyped amniocytes or maternal WBCs via G-banding analysis of metaphase chromosome spreads at 450-band resolution. |
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Structural aberration: a change in chromosome structure detectable by microscopic examination of the metaphase stage of cell division, observed as deletions and fragments, intrachanges or interchanges. |
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Fertilization rate was defined as the number of oocytes with two pronuclei divided by the total number of mature metaphase II oocytes. |
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It has been established that, cumulus cells have an important role in oocyte meiotic transition from prophase to metaphase. |
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This phenomenon was studied by mitosis inhibition assay using onion root tip assay. 777 Oil arrest the cell division especially during the mid anaphase to metaphase stage. |
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Likewise, a checkpoint during mitosis ensures that the cell's spindle fibres are properly aligned in metaphase before the chromosomes are separated in anaphase. |
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Following subcutaneous implantation of bromodeoxyuridine and 26 hours after chlorine dioxide administration, approximately 25 bone marrow metaphase cells from each animal were assessed for sister chromatid exchange. |
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Using fluorescently labelled BACs selected from this physical map in chromosomal FISH experiments, we allocated these physical map assemblies to specific chromosomes on metaphase spreads. |
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Unlike of prophase number of metaphase is reduced in all experimental treatments. |
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Figure 3E shows an example of metaphase spread and its karyogram of a heterozygous diploid. |
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These stages are prophase, prometaphase, metaphase, anaphase, and telophase. |
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Prior to the current study, the known mitotic checkpoints monitored the proper completion of metaphase before allowing the process to proceed to anaphase. |
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After 13-14 hr, mice were sacrificed by cervical dislocation and oocytes at the second metaphase of meiosis were collected from oviductal ampullae. |
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This is because scoring of unbanded chromosomes in metaphase preparations to detect aberrations is labor intensive and prone to technical artifacts. |
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Ultrastructure of meiotic kinetochores investigated by electron microscopy revealed that bivalents in metaphase I have defined kinetochore region for each chromatid. |
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