The mixture was gently pipetted and then centrifuged at 1000 rpm for 10 minutes. |
After 24 h the contents of the container were stirred and 25 ml was pipetted into test tubes and freeze-dried. |
These were shaken for 1 hour, then the solutions were centrifuged and the clear supernatant was pipetted into vials. |
Ten minutes after adding malate, we again pipetted the supernatant from the same dish into other test tubes. |
After incubation, 5 ml of RPMI media were added to the cell flasks and then the cell solution was pipetted to a 50-ml vial. |
Latex beads pipetted onto the surface of adherent aggregates attach to the membrane and are internalized. |