| The ligated mRNA is reverse transcribed using a general or gene-specific primer. |
| The cystic artery is ligated and transected and the gallbladder is dissected from the gallbladder fossa and the right hepatic lobe. |
| Fucose was ligated to bovine serum albumin and antibodies were raised against the conjugate. |
| The cut DNAs were ligated at low concentrations, to encourage intramolecular ligation reactions. |
| These fragments were ligated to the vector of approximately the same molarity to minimize tandem inserts in one clone. |
| The inserts were ligated into the plasmid vector and primary transformants were generated as per the supplied protocol. |
