The presented study has revealed single-molecule dissociation data for RNA molecules of increasing structural complexity. |
The content of RNA in the solution was determined by ultraviolet spectrophotometric analysis. |
Prions do not contain DNA or RNA as do fungi, bacteria, viruses, viroids, or any other previously known infectious entities. |
The helical segments were presumed to be elements of stem-loop structures formed when the RNA was condensed and encapsidated by the protein. |
During translocation the elongation complex moves along the DNA double helix carrying an RNA chain of increasing length. |
In the third step, an RNA oligo that carries two primer sites is ligated to the decapped mRNA by T4 RNA ligase. |