Then, we fix the adsorbed DNA molecules in their conformations thanks to uranyl acetate and we dry the sample using filter paper. |
At some point, increasing error causes major information loss because many conformations populate the average noise sphere. |
We then estimate the free energies of all dimer conformations in all-atom molecular mechanics simulations with explicit water. |
The stereochemistry of the model was checked and all residues found to be in allowed conformations. |
For example, oxytocin and vasopressin have about a thousand different conformations in solution, all in dynamic equilibrium one with another. |
Analysis of protein loop conformations is a notoriously difficult task because they do not have easily identifiable regular geometric patterns. |